Shiro Ishii

committed numerous atrocities beginning in 1936: Vivisection of living people (including pregnant women who were impregnated by the doctors), prisoners had limbs amputated and reattached to other parts of their body, parts of bodies were frozen and thawed to study the resulting untreated gangrene. Humans were also used as living test cases for grenades and flame throwers. Prisoners were injected with inoculations of disease, disguised as vaccinations, to study their effects.